AFB1
Aflatoxin B1-degrading enzyme
Acts on the 8,9-enol ether bond in the difuran toxic structure of aflatoxin B1, oxidizing and then degrading aflatoxin B1 into non-toxic substances.
SIX MYCOTOXINS · ONE ENZYME BLEND
ADZ Toxizyme
A Plus
Pure native enzymes. True degradation.
True Zhendujie. True detoxification.
New product launch
A compound blend of six mycotoxin-degrading enzymes for food safety and animal production.
Explore the technology ↗
National High-Tech EnterpriseNational Science and Technology SMEHACCP System Certified EnterpriseISO9001 Quality Management System Certified EnterpriseFeed Enzyme Preparation Engineering Technology Research Center
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Research translated into practical solutions
Zhendujie (Hangzhou) Biotechnology Engineering Co., Ltd. is engaged in mycotoxin research and development, testing, and the commercialization of research achievements. The company holds leading technologies for deoxynivalenol-degrading enzymes and microorganisms, as well as multiple invention patents. Since 2015, it has carried out collaborative research and experiments with Hangzhou Zhendujie Research Institute, Zhejiang University of Science and Technology, Zhejiang University of Technology, China Agricultural University, Huazhong Agricultural University, Henan Agricultural University, and university joint mycotoxin laboratories. Working with more than 30 PhD researchers, it has developed six food-grade mycotoxin-degrading enzymes and enzymes that inhibit and break down fungi, for use in food safety and livestock production. In 2024, together with the joint laboratory, it published a new method for detecting deoxynivalenol in food on the official Nature website, a globally authoritative academic platform.
Six functions in one
Aflatoxin B1-degrading enzyme, zearalenone-degrading enzyme, deoxynivalenol-degrading enzyme, T-2 toxin-degrading enzyme, fumonisin-degrading enzyme, ochratoxin-degrading enzyme, and related enzymes.
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Application-specific solutions
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Six targeted degradation mechanisms
ZEN
Zearalenone-degrading enzyme
Acts on the lactone bond in the toxic structure of zearalenone, hydrolyzing zearalenone into substances of lower toxicity.
DON
Deoxynivalenol-degrading enzyme
Acts on the hydroxyl group at the C3 position in the toxic structure of deoxynivalenol, oxidizing it into the less toxic 3-keto-DON and reducing toxicity by more than 90%.
T-2
T-2 toxin-degrading enzyme
Uses acetyl coenzyme A as a co-substrate to catalyze the conversion of T-2 toxin into 3-acetyl-T-2 toxin.
FB1
Fumonisin-degrading enzyme
Acts on the ester bond of fumonisin B1 to produce hydrolyzed fumonisin B1 with lower toxicity.
OTA
Ochratoxin-degrading enzyme
Acts on the amide bond of OTA to produce non-toxic OTα and phenylalanine.
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Enzyme activity after exposure to 85°C
In the 85°C high-temperature experiment, all three degrading enzymes were damaged to varying degrees, but still showed good degradation performance.
| Enzyme | Temperature | Exposure | Activity retained |
|---|---|---|---|
| AFB1-degrading enzyme | 85°C | 5 min | 83.9% |
| ZEN-degrading enzyme | 85°C | 5 min | 88.5% |
| DON-degrading enzyme | 85°C | 5 min | 82.7% |
Conclusion: ADZ Toxizyme A Plus tolerates high temperatures with limited enzyme activity loss and can be used in pelleted feed.
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Third-party testing & degradation rates
| Mycotoxin | Reaction time | Degradation rate |
|---|---|---|
| Aflatoxin B1 | 1.5 hours | More than 99% |
| Zearalenone | 2 hours | 100% |
| Deoxynivalenol | 4 hours | More than 90% |
| Ochratoxin | 2 hours | More than 90% |
| T-2 toxin | 4 hours | More than 80% |
| Fumonisin | 4 hours | More than 85% |
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Patents & system certifications
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Effects of mycotoxins on livestock & poultry
| Mycotoxin | Harm to livestock | Harm to poultry |
|---|---|---|
| Deoxynivalenol (DON) | Damages the digestive system; shedding of gastrointestinal mucosa; reduced feed intake, cessation of eating, vomiting, and feed refusal. | Damages the digestive tract, with lesions in the proventriculus, gizzard, and intestines; reduced feed intake, feed refusal, regurgitation of water, and reduced egg production. |
| Aflatoxin | Primarily damages the liver, interferes with kidney function, injures the digestive system, and inhibits protein synthesis; hepatitis, hemorrhagic necrosis, and bile duct proliferation. Growth retardation, jaundice, rough coat, dead skin and eye discharge, tear stains, depression, and anorexia. | Liver damage; atrophy of the bursa of Fabricius and thymus; reduced immunity and increased susceptibility to disease; vaccine failure; reduced egg weight, fertility, and hatchability. |
| Zearalenone (ZEN) | Estrogenic syndrome and impaired reproductive performance; particularly harmful to breeding animals. In females: abortion, stillbirth, redness and swelling of the mammary glands and vulva, rectal prolapse, and very high mortality. Reduced semen quality in boars. | Shortened peak laying period, reduced egg production and egg quality; frequent ascites and salpingitis; rectal prolapse and swelling of soft tissues around the vent. |
| T-2 toxin | Damages the digestive tract and bone marrow, with stomach and intestinal lesions; reduced feed intake, oral ulcers, skin lesions, anemia, feed refusal, vomiting, neurological disorders, and immunosuppression. | Reduced egg production, poor feather growth, oral ulcers, reduced feed intake, feed refusal, neurological disorders, and suppressed immunity. |
| Fumonisin | Damages the nervous system; impaired growth, pulmonary edema, reduced feed intake, impaired reproduction of lymphoblasts, urinary urgency and frequency, and immunosuppression. | Acute enteritis, black lungs, nephritis, renal calcification, liver dysfunction, reduced feed intake, and immunosuppression. Digestive tract damage produces characteristic black and oily droppings. |
| Ochratoxin | Attacks the kidneys, immune system, and hematopoietic system; the liver becomes fragile; kidney lesions and growth retardation. | Suppresses the kidneys, immune system, and hematopoietic system; incomplete calcium and phosphorus absorption, fragile bones, incomplete eggshell calcification, and a high rate of broken eggs. |
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Comparison with conventional mycotoxin binders
| Category | Physical adsorption | Microbial degradation | Single mycotoxin-degrading enzyme | ADZ Toxizyme A Plus (compound degrading enzymes) |
|---|---|---|---|---|
| Main ingredients | 1. Activated carbon. 2. Aluminosilicates: montmorillonite, bentonite, etc. 3. Yeast cell walls and extracts. | 1. Bacillus subtilis ANSB060. 2. Saccharomyces cerevisiae. 3. Lactobacillus acidophilus. | A single mycotoxin-degrading enzyme. | Aflatoxin B1-, zearalenone-, deoxynivalenol-, T-2 toxin-, fumonisin-, and ochratoxin-degrading enzymes, etc. |
| Advantages | 1. High adsorption efficiency for AFB1. 2. Low price and easy availability. 3. Simple in vitro evaluation with evident effects. | 1. Some degradation effect. 2. Degrades different mycotoxins. 3. Does not adsorb small-molecule nutrients in feed. | 1. Clearly identified degradation products and a high safety factor. 2. Strong specificity and high degradation efficiency. 3. Does not damage feed nutrients. 4. No environmental pollution; significant effects in animal experiments. | Scientifically formulated multiple specific enzymes act on multiple targets simultaneously to break down several major mycotoxins. |
| Disadvantages | 1. Unstable adsorption; desorption can occur. 2. Poor adsorption of weakly polar toxins such as ZEN, DON, OTA, and FB1. 3. Adsorbs feed nutrients and disrupts nutritional balance. 4. Adsorbed toxins are excreted and pollute the environment. 5. High inclusion rates occupy space in the feed formula. | 1. Microorganisms entering the gut consume feed nutrients. 2. Complex toxin metabolites raise safety issues. 3. In practical use, effects still rely on microbial adsorption. 4. Adsorbents are used in product carriers. | 1. Degrades only one toxin type. 2. Blending is required according to the levels of different toxins. 3. Low inclusion rates require premixing. | The unit selling price is higher than that of ordinary adsorbents; long-term overall use costs are lower than those of combining multiple solutions. |
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Directions & packaging
10 gper tonne of complete feed
5 gper tonne of drinking water
Adjust the dosage as appropriate according to feed quality and seasonal conditions.
Pack sizes
1. 5 kg per package.
2. 1 kg × 10 per package.
